Guideline:
HIV-related hematologic manifestations in pediatrics
Bibliographic Source(s)
- New York State Department of Health. HIV-related hematologic manifestations in pediatrics. New York (NY): New York State Department of Health; 2003. 12 p. [14 references]
Guideline Status
This is the current release of the guideline.
Guideline Category
Diagnosis
Evaluation
Management
Screening
Treatment
Intended Users
Health Care Providers
Physician Assistants
Physicians
Public Health Departments
Guideline Objective(s)
To develop guidelines to reduce human immunodeficiency virus (HIV)-related hematologic manifestations in pediatric patients
Target Population
Human immunodeficiency virus (HIV)-infected children
Interventions and Practices Considered
Screening
- Complete blood count with differential every 3 months; more frequent complete blood counts as indicated in the guideline
- Lead levels every year and when toxicity is suspected
Diagnosis/Evaluation – refer to Major Recommendations for context
Anemia
- Nutritional history
- Review of systems (especially the gastrointestinal tract)
- Iron studies specifically serum iron total iron-binding capacity transferrin saturation and serum ferritin
- Hemoglobin electrophoresis with quantitative measurement of hemoglobin A2 and hemoglobin F if indicated
- Evaluation for hemolysis (e.g. direct Coombs test serum lactate dehydrogenase medication list splenomegaly)
- Exclude opportunistic infection: cultures bone marrow examination
- Liver and thyroid function tests vitamin B12 and folate levels bone marrow aspiration and biopsy
Thrombocytopenia
- Physical examination especially organomegaly or lymphadenopathy
- Consideration of non-platelet components of the complete blood count
- Consideration of response to platelet-directed treatment
- Bone marrow examination in consultation with specialist when malignancy is suspected
Coagulation Abnormalities
Preoperative evaluation of bleeding tendencies by medical history physical examination and basic hemostatic tests (e.g. partial thromboplastin time [PTT] prothrombin time [PT] fibrinogen platelet count and thrombin time) if indicated
Treatment
Anemia
- Erythropoietin therapy with supplemental oral iron and folate
- Blood transfusion with irradiated and leukocyte-reduced blood products
Thrombocytopenia
- Antiretroviral therapy
- Intravenous anti-Rho immunoglobulin (IV anti-D) with premedications; intravenous immunoglobulin as an alternative
- Prednisone (after malignancy excluded)
Neutropenia
Granulocyte-colony stimulating factor (G-CSF)
Coagulation Abnormalities
- Fresh frozen plasma (FFP)
- Cryoprecipitate
- Desmopressin
- Antifibrinolytics (e.g. epsilon aminocaproic acid [EACA])
Major Outcomes Considered
Not stated
Methods Used to Collect/Select Evidence
Hand-searches of Published Literature (Primary Sources)
Hand-searches of Published Literature (Secondary Sources)
Searches of Electronic Databases
Description of Methods used to Collect/Select the Evidence
Not stated
Number of Source Documents
Not stated
Methods Used to Assess the Quality and Strength of the Evidence
Expert Consensus (Committee)
Rating Scheme for the Strength of the Evidence
Not applicable
Methods Used to Analyze the Evidence
Review
Description of the Methods Used to Analyze the Evidence
Not applicable
Methods Used to Formulate the Recommendations
Expert Consensus
Description of Methods Used to Formulate the Recommendations
The Human Immunodeficiency Virus (HIV) Guidelines Program works directly with committees composed of HIV Specialists to develop clinical practice guidelines. These specialists represent different disciplines associated with HIV care including infectious diseases family medicine obstetrics and gynecology among others. Generally committees meet in person 3-4 times per year and otherwise conduct business through monthly conference calls.
Committees meet to determine priorities of content review literature and weigh evidence for a given topic. These discussions are followed by careful deliberation to craft recommendations that can guide HIV primary care practitioners in the delivery of HIV care. Decision making occurs by consensus. When sufficient evidence is unavailable to support a specific recommendation that addresses an important component of HIV care the group relies on their collective best practice experience to develop the final statement. The text is then drafted by one member reviewed and modified by the committee edited by medical writers and then submitted for peer review.
Rating Scheme for the Strength of the Recommendations
Not applicable
Cost Analysis
A formal cost analysis was not performed and published cost analyses were not reviewed.
Method of Guideline Validation
Peer Review
Description of Method of Guideline Validation
Not stated
Major Recommendations
Screening for Hematologic Abnormalities
A complete blood count with differential should be performed every 3 months to screen for hematologic abnormalities in human immunodeficiency virus (HIV)-infected children. Complete blood count may need to be obtained more often for children receiving bone marrow suppressive therapy or if abnormalities are identified.
Anemia
Types of Anemia
Microcytic Anemia
The diagnostic evaluation for microcytic anemia in the HIV-infected child should include a careful nutritional history review of systems (especially the gastrointestinal tract) and iron studies specifically serum iron total iron-binding capacity transferrin saturation and serum ferritin. If indicated by family history or if the anemia persists after a therapeutic trial of iron hemoglobin electrophoresis with quantitative measurement of hemoglobin A2 and hemoglobin F should be performed.
Screening of lead levels should be performed yearly in all children and whenever toxicity is suspected.
Normocytic Anemia
Hemolytic Anemia
Evaluation for hemolysis should be performed in individuals with unusually high transfusion requirements or those with high reticulocyte count low serum haptoglobin (only for intravascular hemolysis) presence of microspherocytes on the peripheral smear indirect hyperbilirubinemia or bone marrow erythroid hyperplasia.
Hypoplastic Anemia
If a patient presents with hypoplastic anemia in the presence of fever weight loss or new physical findings opportunistic infections should be excluded. Appropriate cultures and bone marrow examination should be performed to help establish the diagnosis.
Macrocytic Anemia
If megaloblastic anemia cannot be explained by common causes such as medications including antiretroviral (ARV) drugs evaluation should include liver and thyroid function tests vitamin B12 and folate levels bone marrow aspiration and biopsy to evaluate for the possibility of bone marrow failure or myelodysplasia.
Treatment of Anemia
If endogenous erythropoietin levels are <500 mUnits/mL erythropoietin therapy (50-200 iu/kg/dose 3 times/week) should be administered to reduce the need for transfusion. Supplemental oral iron (3-6 mg/kg/day of elemental iron) and folate (1 mg/day) should be administered when erythropoietin is initiated.
If clinically significant anemia (i.e. hemoglobin <7 g/dL or cardiorespiratory compromise) develops within the first month of life and zidovudine prophylaxis the use of erythropoietin or transfusion is recommended to allow sustained use of zidovudine until the diagnosis of perinatal infection has been established. The zidovudine dose should not be modified.
If severe anemia develops after the fourth week of zidovudine prophylaxis zidovudine may be discontinued at that time rather than subjecting the neonate to blood transfusion or erythropoietin.
The necessity for blood transfusion should be evaluated carefully. Transfusions should be reserved for clinically significant severe anemia. Irradiation and leukocyte reduction of blood according to standard protocols should be used for all transfusions.
Thrombocytopenia
Pathophysiology and Diagnosis
If thrombocytopenia is identified the differential diagnosis should be established according to the presence or absence of one or more of the following:
- abnormalities in the physical examination especially organomegaly or lymphadenopathy
- abnormalities in the non-platelet components of the complete blood count
- failure to respond to platelet-directed treatment
If physical examination abnormalities or multiple cell line deficits are present or if the platelet count does not respond to platelet-directed treatment prompt investigation for infectious toxic or malignant causes should be performed.
A bone marrow examination should be performed in consultation with a hematologist/ oncologist when malignancy is suspected.
If thrombocytopenia is accompanied by other cytopenias or splenomegaly and is mild (>50000 cells/mm3) hypersplenism caused by infectious causes or coincident liver disease should be suspected.
Treatment of Thrombocytopenia
Antiretroviral therapy should be the primary treatment of HIV-associated thrombocytopenic purpura unless 1) it has been previously demonstrated to be ineffective 2) the count needs to be increased within 2 weeks or 3) there are other reasons not to initiate it such as refusal intolerance or limited antiretroviral susceptibility. Treatment of asymptomatic mild to moderate HIV-associated thrombocytopenia is usually not necessary. When the platelet counts are <20000 to 30000 cells/mm3 treatment should be initiated in consultation with a hematologist. Treatment should be initiated in patients with bleeding tendencies such as hemophilia when the platelet count is <50000 cells/mm3.
For most patients who need treatment for HIV-associated thrombocytopenia the treatment of choice is intravenous anti-Rho immunoglobulin (IV anti-D) 50 micrograms/kg with premedications.
For patients unable to receive anti-D because they are either Rh(–) DAT(+) or have undergone a splenectomy intravenous immunoglobulin (1 g/kg) is the next best treatment. Prednisone has also been effective in treating thrombocytopenia and may be administered once malignancy has been ruled out.
Neutropenia
Treatment of Neutropenia
If neutropenia is confirmed to be persistent and severe (<500 cells/mm3) rather than transient consideration should be given to instituting granulocyte-colony stimulating factor (G-CSF). The initial dose is 5 micrograms/kg/day given subcutaneously. The G-CSF dosing required varies greatly from person to person; dosing frequency should be titrated to an individual's response. In children with multiple cell line deficits G-CSF may exacerbate thrombocytopenia; therefore platelet counts should be monitored. Bone marrow aspiration before initiating G-CSF therapy is not necessary unless there is also evidence of anemia thrombocytopenia new lymphadenopathy or hepatosplenomegaly.
Coagulation Abnormalities
Diagnosis
The preoperative evaluation of bleeding tendency should include a medical history physical examination and if indicated basic hemostatic screening tests. The information obtained in the medical history should include the presence of abnormal bruising both extensive or unexplained; gum bleeding; prolonged bleeding after laceration or surgery such as circumcision tonsillectomy tooth extractions or biopsies; epistaxis; menorrhagia; hematuria; and melena in the patient and in the family. In addition information regarding liver or renal disease or changes in medication in the HIV-infected patient should be obtained.
Routine pre-operative bleeding screening tests such as a partial thromboplastin time (PTT) prothrombin time (PT) fibrinogen platelet count and thrombin time should be reserved for patients with a positive assessment by history or those with a negative history who will be undergoing surgeries with a high risk of bleeding such as tonsillectomy central nervous system surgery cardiac surgery or scoliosis repair.
When a prolonged PTT is present mixing studies (1:1 dilution with normal plasma) should be obtained. Failure to correct a prolonged PTT should be indicative of the presence of antiphospholipid antibodies (aPL) or specific factor inhibitors. If the mixing studies reveal correction assays for factors VIII IX XI and XII should be performed.
Primary hemostasis tests including von Willebrand factor (vWF) studies platelet aggregation factors VIII IX XI XIII alpha 2-antiplasmin and plasminogen activator inhibitor (PAI) should be obtained for patients with normal PT PTT fibrinogen and platelet count who are at high risk for bleeding based on history. Neither the bleeding time nor the Platelet Function Analyzer (PFA)-100 is recommended because of the absence of data to support that these tests predict bleeding.
Treatment of Coagulation Abnormalities
Treatment of HIV-associated coagulation abnormalities should be based on the specific diagnosis as well as bleeding history (see Table below).
Table: General Therapeutic Options for Treating Coagulation Abnormalities in HIV-infected Children
| Coagulation Abnormality | Therapy |
|---|---|
|
Fresh frozen plasma (FFP 10-20 mL/kg) |
|
Cryoprecipitate (1 bag/10 lb) |
|
Desmopressin (refer to the original guideline document for dosing information) |
|
Antifibrinolytics [e.g. epsilon aminocaproic acid (EACA 100 mg/kg po or IV)] |
Clinical Algorithm(s)
None provided
Type of Evidence supporting the Recommendations
The type of evidence supporting the recommendations is not stated.
Potential Benefits
Appropriate management of HIV-related hematologic manifestations in pediatrics including anemia thrombocytopenia neutropenia and coagulation abnormalities.
Potential Harms
- Risks associated with red cell transfusion. The indications risks and benefits for red cell transfusions are similar to those of non-human immunodeficiency virus (HIV)-infected patients. However when transfusing the HIV-infected patient there are several special concerns that predate the availability of routine leuko-reduction. Studies have shown increased viral load 5 days after the transfusion increased incidence of cytomegalovirus infection and death in patients with advanced disease although most of these studies were conducted in adults. The survival rate is possibly decreased in patients who are given transfusions compared with patients who have similar degrees of anemia and immunodeficiency who are not transfused. Irradiation and leukocyte reduction have greatly reduced the risk of infection and graft-versus-host disease related to transfusion.
- Adverse effects of intravenous anti-Rho immunoglobulin (IV anti-D). Potential adverse effects of anti-D can include fever headache chills signs of hypersensitivity including anaphylaxis or signs of intravascular hemolysis.
- Risks associated with steroids. Although the response of HIV-infected patients to steroids is comparable to that of uninfected patients it is not sustained in 60% to 80% of patients unless it is continued at a high dose which may however increase the risk for secondary infections such as Candida and Aspergillus infections.
- Adverse effects of granulocyte-colony stimulating factor (G-CSF). Toxicity is rare but includes increase in spleen size bone pain and leukocytoclastic vasculitis. G-CSF may also exacerbate thrombocytopenia.
Contraindications
Antifibrinolytics are contraindicated in patients with genitourinary bleeding.
Description of Implementation Strategy
Following the development and dissemination of guidelines the next crucial steps are adoption and implementation. Once practitioners become familiar with the content of guidelines they can then consider how to change the ways in which they take care of their patients. This may involve changing systems that are part of the office or clinic in which they practice. Changes may be implemented rapidly especially when clear outcomes have been demonstrated to result from the new practice such as prescribing new medication regimens. In other cases such as diagnostic screening or oral health delivery however barriers emerge which prevent effective implementation. Strategies to promote implementation such as through quality of care monitoring or dissemination of best practices are listed and illustrated in the companion document to the original guideline (HIV clinical practice guidelines New York State Department of Health; 2003) which portrays New York's HIV Guidelines Program. The general implementation strategy is outlined below.
- Statement of purpose and goal to encourage adoption and implementation of guidelines into clinical practice by target audience.
- Define target audience (providers consumers support service providers)
- Are there groups within this audience that need to be identified and approached with different strategies? (e.g. HIV Specialists family practitioners minority providers professional groups rural-based providers)
- Define implementation methods
- What are the best methods to reach these specific groups (e.g. performance measurement consumer materials media conferences)?
- Determine appropriate implementation processes
- What steps need to be taken to make these activities happen?
- What necessary processes are internal to the organization (e.g. coordination with colleagues monitoring of activities)?
- What necessary processes are external to the organization (e.g. meetings with external groups conferences)?
- Are there opinion leaders that can be identified from the target audience that can champion the topic and influence opinion?
- Monitor Progress
- What is the flow of activities associated with the implementation process and which can be tracked to monitor the process?
- Evaluate
- Did the processes and strategies work? Were the guidelines implemented?
- What could be improved in future endeavors?
Implementation Tools
Personal Digital Assistant (PDA) Downloads
For information about availability see the "Availability of Companion Documents" and "Patient Resources" fields below.
IOM Care Need
Living with Illness
IOM Domain
Effectiveness
Bibliographic Source(s)
- New York State Department of Health. HIV-related hematologic manifestations in pediatrics. New York (NY): New York State Department of Health; 2003. 12 p. [14 references]
Adaptation
Not applicable: The guideline was not adapted from another source.
Source(s) of Funding
New York State Department of Health
Guideline Committee
Committee for the Care of Children and Adolescents with HIV Infection
Composition of Group that Authored the Guideline
Committee Chair: Joseph S. Cervia MD Director The Comprehensive HIV Care and Research Center Long Island Jewish Medical Center
Committee Vice Chair: Jeffrey M. Birnbaum MD MPH Director HEAT Program Kings County Hospital
Committee Members: Elaine Abrams MD Director Family Care Center Department of Pediatrics Harlem Hospital Center; Saroj Bakshi MD Chief Division of Pediatric Infectious Diseases Bronx-Lebanon Hospital Center; Howard J. Balbi MD Director Pediatric Infectious Diseases and Pediatric AIDS Program Nassau County Medical Center; Coleen K. Cunningham MD Associate Professor of Pediatrics SUNY Upstate Medical University; Samuel Grubman MD Chief Allergy and Immunology Saint Vincents Catholic Medical Centers St. Vincent's Manhattan; Sharon Nachman MD Chief Pediatric Infectious Diseases Associate Professor of Pediatrics SUNY at Stony Brook. Department of Pediatrics; Catherine J. Painter MD PhD Assistant Professor of Clinical Pediatrics College of Physicians and Surgeons Columbia University Medical Director Incarnation Children's Center; Vicki Peters MD Coordinator Pediatric HIV Special Projects Office of AIDS Surveillance New York City Department of Health; Roberto Posada MD Assistant Professor of Pediatrics Division of Pediatric Infectious Diseases Director Pediatric HIV Program Mount Sinai School of Medicine; Barbara Warren BSN MPH PNP Assistant Bureau Director Bureau of HIV Ambulatory Care Services AIDS Institute New York State Department of Health; Geoffrey A. Weinberg MD Director Pediatric HIV Program Associate Professor of Pediatrics Department of Pediatrics University of Rochester School of Medicine and Dentistry; Ed Handelsman MD Assistant Professor of Pediatrics SUNY Health Sciences Center at Downstate Assistant Medical Director of Pediatrics Office of the Medical Director AIDS Institute
Financial Disclosures/Conflicts of Interest
Not stated
Guideline Status
This is the current release of the guideline.
Guideline Availability
Electronic copies: Available from the New York State Department of Health AIDS Institute Web site.
Print copies: Available from Office of the Medical Director AIDS Institute New York State Department of Health 5 Penn Plaza New York NY 10001; Telephone: (212) 268-6108.
Availability of Companion Documents
The following is available:
- HIV clinical practice guidelines. New York (NY): New York State Department of Health; 2003. 36 p.
Electronic copies: Available from the New York State Department of Health AIDS Institute Web site.
Print copies: Available from Office of the Medical Director AIDS Institute New York State Department of Health 5 Penn Plaza New York NY 10001; Telephone: (212) 268-6108.
This guideline is available as a Personal Digital Assistant (PDA) download from the New York State Department of Health AIDS Institute Web site.
Patient Resources
None available
NGC STATUS
This summary was prepared by ECRI on January 21 2004. This summary was updated by ECRI on January 31 2006 following release of a public health advisory from the U.S. Food and Drug Administration regarding the use of WinRho SDF (Rho(D) Immune Globulin Intravenous [Human]). This summary was updated by ECRI on January 29 2007 following the U.S. Food and Drug Administration advisory on erythropoiesis stimulating agents. This summary was updated by ECRI Institute on July 9 2007 following the FDA advisory on erythropoiesis stimulating agents. This summary was updated by ECRI Institute on December 7 2007 following the U.S. Food and Drug Administration advisory on Desmopressin Acetate. This summary was updated by ECRI Institute on March 21 2008 following the FDA advisory on Erythropoiesis Stimulating Agents. This summary was updated by ECRI Institute on August 15 2008 following the U.S. Food and Drug Administration advisory on Erythropoiesis Stimulating Agents (ESAs).
COPYRIGHT STATEMENT
This NGC summary is based on the original guideline which is copyrighted by the guideline developer. See the New York State Department of Health AIDS Institute Web site for terms of use.
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