Nanobody-based biosensors promise exceptional molecular recognition and robustness, but their implementation has been limited by unstable and non-scalable surface chemistries. Here we introduce a materials platform that integrates stable electropolymerized tyramine nanofilms with label-free, non-faradaic electrochemical impedance spectroscopy for direct molecular detection. The electropolymerized nanofilms form amine-functionalized coatings that are electrically insulating yet chemically active, supporting site-specific covalent immobilization of nanobodies in controlled orientations. Target binding induces more than a 50% increase in the capacitance signal response, providing a distinct label-free signature of molecular recognition. The platform can be adapted to diverse bioreceptors and antifouling layers, offering a general route to chemically robust and scalable bioelectronic interfaces. By decoupling film conductivity from functional stability, this work establishes a new class of interfaces that bridge molecular design with label-free signal transduction for next-generation biosensing technologies.
Nanoscale
Journal Article
English
41996663
Guideline Central and select third party use “cookies” on this website to enhance the user experience.
This technology helps us gather statistical and analytical information to optimize the relevant content for you.
The user also has the option to opt-out which may have an effect on the browsing experience.