COVID-19 Guideline Part 3: Molecular Testing

Authoring Organization

Publication Month/Year

September 5, 2023

Last Updated Month/Year

February 7, 2024

Document Type


Country of Publication


Document Objectives

The IDSA’s goal was to develop an evidence-based diagnostic guideline to assist clinicians, clinical laboratorians, patients and policymakers in decisions related to the optimal use of SARS-CoV-2 nucleic acid amplification tests. In addition, we provide a conceptual framework for understanding molecular diagnostic test performance, discuss the nuance of test result interpretation in a variety of practice settings and highlight important unmet research needs in the COVID-19 diagnostic testing space.

Inclusion Criteria

Male, Female, Adolescent, Adult, Child, Older adult

Health Care Settings

Ambulatory, Hospital, Laboratory services

Intended Users

Epidemiology infection prevention, healthcare business administration, laboratory technician, nurse, nurse practitioner, physician, physician assistant


Diagnosis, Assessment and screening

Diseases/Conditions (MeSH)

D000086382 - COVID-19, D000087123 - COVID-19 Nucleic Acid Testing, D000086742 - COVID-19 Testing


molecular testing, Coronavirus, covid-19, COVID

Supplemental Methodology Resources

Data Supplement